Performance Comparison of Static and Rotating Bioreactor for Bacterial Cellulose Production from Tofu Wastewater
DOI:
https://doi.org/10.33096/jcpe.v11i1.2362Keywords:
microbial cellulose, waste-to-value, Acetobacter, whey tofu, nata de soyaAbstract
Tofu industry wastewater is a potential substrate for bacterial cellulose (BC) production due to its residual nutrient content. However, conventional static culture has limited oxygen transfer and substrate diffusion, resulting in low substrate utilization efficiency. This study presents a comparative evaluation of static and rotating bioreactor (RB) systems, with a primary focus on substrate utilization, bacterial growth, and BC yield. In addition, the effect of inoculum volume (10% and 30%) was investigated to assess its influence on process performance. The results demonstrate that the RB system significantly outperforms static culture, achieving 1.6 – 2 times higher substrate utilization and 53.3 - 61.3% higher BC yield on initial substrate. The highest BC product is 5 g dry mass BC/ L medium in the RB system. Furthermore, the RB system enhances substrate conversion toward bacterial growth, indicating improved metabolic efficiency under dynamic conditions. A lower inoculum volume, 10% inoculum with 1-10 million CFU/mL, resulted in better overall performance compared to 30% inoculum, suggesting that excessive inoculum does not improve process efficiency. These findings confirm that the rotating bioreactor is a more effective system for intensifying BC production, particularly in terms of substrate utilization, and highlight its potential for scalable and sustainable bioprocess applications.
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[1] M. Margono, N. Isnaeni, V. Amelia, and E. N. Shohih, ‘Process of Bacterial Cellulose Production from Tofu Wastewater Without Pretreatment Using Acetobacter xylinum’, Jurnal Bahan Alam Terbarukan, vol. 12, no. 2, pp. 129–136, Dec. 2023.
[2] X. Feng, Z. Ge, Y. Wang, X. Xia, B. Zhao, and M. Dong, ‘Production and characterization of bacterial cellulose from kombucha-fermented soy whey’, Food Prod Process and Nutr, vol. 6, no. 1, p. 20, Feb. 2024, doi: 10.1186/s43014-023-00188-3.
[3] S. Taokaew, ‘Bacterial Nanocellulose Produced by Cost-Effective and Sustainable Methods and Its Applications: A Review’, Fermentation, vol. 10, no. 6, p. 316, Jun. 2024, doi: 10.3390/fermentation10060316.
[4] M. O. Akintunde, B. C. Adebayo-Tayo, M. M. Ishola, A. Zamani, and I. S. Horváth, ‘Bacterial Cellulose Production from agricultural Residues by two Komagataeibacter sp. Strains’, Bioengineered, vol. 13, no. 4, pp. 10010–10025, Apr. 2022, doi: 10.1080/21655979.2022.2062970.
[5] I. de A. A. Fernandes et al., ‘Bacterial cellulose: From production optimization to new applications’, International Journal of Biological Macromolecules, vol. 164, pp. 2598–2611, Dec. 2020, doi: 10.1016/j.ijbiomac.2020.07.255.
[6] S.-S. Wang et al., ‘Physicochemical characterization of high-quality bacterial cellulose produced by Komagataeibacter sp. strain W1 and identification of the associated genes in bacterial cellulose production’, RSC Adv., vol. 7, no. 71, pp. 45145–45155, Sep. 2017, doi: 10.1039/C7RA08391B.
[7] V.-D. Girard, J. Chaussé, and P. Vermette, ‘Bacterial cellulose: A comprehensive review’, Journal of Applied Polymer Science, vol. 141, no. 15, p. e55163, 2024, doi: 10.1002/app.55163.
[8] I. Orlando, P. Basnett, R. Nigmatullin, W. Wang, J. C. Knowles, and I. Roy, ‘Chemical Modification of Bacterial Cellulose for the Development of an Antibacterial Wound Dressing’, Front. Bioeng. Biotechnol., vol. 8, Sep. 2020, doi: 10.3389/fbioe.2020.557885.
[9] L. Quijano et al., ‘Bacterial cellulose cookbook: A systematic review on sustainable and cost-effective substrates’, Journal of Bioresources and Bioproducts, p. S2369969824000380, May 2024, doi: 10.1016/j.jobab.2024.05.003.
[10] Z. Liu et al., ‘Efficient Production of Bacterial Cellulose Using Komagataeibacter sucrofermentans on Sustainable Feedstocks’, ChemSusChem, vol. 18, no. 6, p. e202401578, 2025, doi: 10.1002/cssc.202401578.
[11] I. Vigentini et al., ‘Set-Up of Bacterial Cellulose Production From the Genus Komagataeibacter and Its Use in a Gluten-Free Bakery Product as a Case Study’, Front. Microbiol., vol. 10, Sep. 2019, doi: 10.3389/fmicb.2019.01953.
[12] J. Wang, J. Tavakoli, and Y. Tang, ‘Bacterial cellulose production, properties and applications with different culture methods – A review’, Carbohydrate Polymers, vol. 219, pp. 63–76, Sep. 2019, doi: 10.1016/j.carbpol.2019.05.008.
[13] M. A. Cruz, O. Flor-Unda, A. Avila, M. D. Garcia, and L. Cerda-Mejía, ‘Advances in Bacterial Cellulose Production: A Scoping Review’, Coatings, vol. 14, no. 11, p. 1401, Nov. 2024, doi: 10.3390/coatings14111401.
[14] K. Fijałkowski et al., ‘Modification of bacterial cellulose through exposure to the rotating magnetic field’, Carbohydrate Polymers, vol. 133, pp. 52–60, Nov. 2015, doi: 10.1016/j.carbpol.2015.07.011.
[15] R. R. Singhania, A. K. Patel, M.-L. Tsai, C.-W. Chen, and C. Di Dong, ‘Genetic modification for enhancing bacterial cellulose production and its applications’, Bioengineered, vol. 12, no. 1, pp. 6793–6807, Dec. 2021, doi: 10.1080/21655979.2021.1968989.
[16] R. A. H. Almihyawi, E. Musazade, N. Alhussany, S. Zhang, and H. Chen, ‘Production and characterization of bacterial cellulose by Rhizobium sp. isolated from bean root’, Sci Rep, vol. 14, no. 1, p. 10848, May 2024, doi: 10.1038/s41598-024-61619-w.
[17] Y. S. Tseng, A. K. Patel, C.-W. Chen, C.-D. Dong, and R. R. Singhania, ‘Improved production of bacterial cellulose by Komagataeibacter europaeus employing fruit extract as carbon source’, J Food Sci Technol, vol. 60, no. 3, pp. 1054–1064, Mar. 2023, doi: 10.1007/s13197-022-05451-y.
[18] A. K. Wani, N. Akhtar, F. Sher, A. A. Navarrete, and J. H. P. Américo-Pinheiro, ‘Microbial adaptation to different environmental conditions: molecular perspective of evolved genetic and cellular systems’, Arch Microbiol, vol. 204, no. 2, p. 144, Jan. 2022, doi: 10.1007/s00203-022-02757-5.
[19] R. R. Singhania et al., ‘Developments in bioprocess for bacterial cellulose production’, Bioresource Technology, vol. 344, p. 126343, Jan. 2022, doi: 10.1016/j.biortech.2021.126343.
[20] I. Sayah, C. Gervasi, S. Achour, and T. Gervasi, ‘Fermentation Techniques and Biotechnological Applications of Modified Bacterial Cellulose: An Up-to-Date Overview’, Fermentation, vol. 10, no. 2, p. 100, Feb. 2024, doi: 10.3390/fermentation10020100.
[21] M. Bagherniya, V. Babaeipour, and A. Soleimani, ‘Optimization of Bacterial Nano-Cellulose Production in Bench-Scale Rotating Biological Contact Bioreactor by Response Surface Methodology’, Iranian Journal of Chemistry and Chemical Engineering, vol. 40, no. 2, pp. 407–416, Apr. 2021, doi: 10.30492/ijcce.2020.38040.
[22] Y. Zahro, D. A. Nugroho, P. Saroyo, and M. P. Kurniawan, ‘Optimization of bacterial cellulose fermentation medium composition using soybean-boiled wastewater and xylitol: Taguchi design of experiment approach’, IOP Conf. Ser.: Earth Environ. Sci., vol. 1476, no. 1, p. 012085, Apr. 2025, doi: 10.1088/1755-1315/1476/1/012085.
[23] C. Uğurel and H. Öğüt, ‘Optimization of Bacterial Cellulose Production by Komagataeibacter rhaeticus K23’, Fibers, vol. 12, no. 3, p. 29, Mar. 2024, doi: 10.3390/fib12030029.
[24] P. Semjonovs et al., ‘Cellulose synthesis by Komagataeibacter rhaeticus strain P 1463 isolated from Kombucha’, Appl Microbiol Biotechnol, vol. 101, no. 3, pp. 1003–1012, Feb. 2017, doi: 10.1007/s00253-016-7761-8.
[25] J. M. Gonzalez and B. Aranda, ‘Microbial Growth under Limiting Conditions-Future Perspectives’, Microorganisms, vol. 11, no. 7, p. 1641, Jun. 2023, doi: 10.3390/microorganisms11071641.
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